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1u6p

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NMR Structure of the MLV encapsidation signal bound to the Nucleocapsid protein

Structural highlights

1u6p is a 2 chain structure with sequence from Moloney murine leukemia virus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GAG_MLVMS Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release.^[1] Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex (By similarity).^[2] Capsid protein p30 forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex (By similarity).^[3] Nucleocapsid protein p10 is involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved UCUG elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization.^[4]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

↑ Jadwin JA, Rudd V, Sette P, Challa S, Bouamr F. Late domain-independent rescue of a release-deficient Moloney murine leukemia virus by the ubiquitin ligase itch. J Virol. 2010 Jan;84(2):704-15. doi: 10.1128/JVI.01319-09. Epub 2009 Oct 28. PMID:19864377 doi:http://dx.doi.org/10.1128/JVI.01319-09
↑ Jadwin JA, Rudd V, Sette P, Challa S, Bouamr F. Late domain-independent rescue of a release-deficient Moloney murine leukemia virus by the ubiquitin ligase itch. J Virol. 2010 Jan;84(2):704-15. doi: 10.1128/JVI.01319-09. Epub 2009 Oct 28. PMID:19864377 doi:http://dx.doi.org/10.1128/JVI.01319-09
↑ Jadwin JA, Rudd V, Sette P, Challa S, Bouamr F. Late domain-independent rescue of a release-deficient Moloney murine leukemia virus by the ubiquitin ligase itch. J Virol. 2010 Jan;84(2):704-15. doi: 10.1128/JVI.01319-09. Epub 2009 Oct 28. PMID:19864377 doi:http://dx.doi.org/10.1128/JVI.01319-09
↑ Jadwin JA, Rudd V, Sette P, Challa S, Bouamr F. Late domain-independent rescue of a release-deficient Moloney murine leukemia virus by the ubiquitin ligase itch. J Virol. 2010 Jan;84(2):704-15. doi: 10.1128/JVI.01319-09. Epub 2009 Oct 28. PMID:19864377 doi:http://dx.doi.org/10.1128/JVI.01319-09