3bw8

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Crystal structure of the Clostridium limosum C3 exoenzyme

Structural highlights

3bw8 is a 2 chain structure with sequence from Hathewaya limosa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.8Å
Ligands:SO4
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ARC3_HATLI ADP-ribosylates eukaryotic Rho and Rac proteins on an asparagine residue.[UniProtKB:P15879]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

C3-like toxins ADP-ribosylate and inactivate Rho GTPases. Seven C3-like ADP-ribosyltransferases produced by Clostridium botulinum, Clostridium limosum, Bacillus cereus and Staphylococcus aureus were identified and two representatives--C3bot from C. botulinum and C3stau2 from S. aureus--were crystallized. Here we present the 1.8A structure of C. limosum C3 transferase C3lim and compare it to the structures of other family members. In contrast to the structure of apo-C3bot, the canonical ADP-ribosylating turn turn motif is observed in a primed conformation, ready for NAD binding. This suggests an impact on the binding mode of NAD and on the transferase reaction. The crystal structure explains why auto-ADP-ribosylation of C3lim at Arg41 interferes with the ADP-ribosyltransferase activity of the toxin.

Crystal structure of the Clostridium limosum C3 exoenzyme.,Vogelsgesang M, Stieglitz B, Herrmann C, Pautsch A, Aktories K FEBS Lett. 2008 Apr 2;582(7):1032-6. Epub 2008 Mar 4. PMID:18325337[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
2 reviews cite this structure
Clostridial toxins.
Popoff et al. (2009)
1 more
5 other articles
No citations found

See Also

References

  1. ↑ Vogelsgesang M, Stieglitz B, Herrmann C, Pautsch A, Aktories K. Crystal structure of the Clostridium limosum C3 exoenzyme. FEBS Lett. 2008 Apr 2;582(7):1032-6. Epub 2008 Mar 4. PMID:18325337 doi:10.1016/j.febslet.2008.02.051

Contents


PDB ID 3bw8

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