Structural highlights
3c7t is a 4 chain structure with sequence from Bombyx mori. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Method: | X-ray diffraction, Resolution 1.76Å |
| Ligands: | , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
EPP_BOMMO Steroid phosphatase which catalyzes the conversion of inactive phosphorylated ecdysteroids into their active forms (PubMed:12721294, PubMed:15738639, PubMed:18937503). Shows high activity towards ecdysone 22-phosphate (E22P) (PubMed:12721294). Has lower activity towards other ecdysteriod phosphates including 20-hydroxyecdysone 22-phosphate (20E22P) and 2-deoxyecdysone 22-phosphate (2dE22P) (PubMed:12721294). Also has protein tyrosine phosphatase activity (PubMed:18937503).[1] [2] [3]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
References
- ↑ Yamada R, Sonobe H. Purification, kinetic characterization, and molecular cloning of a novel enzyme ecdysteroid-phosphate phosphatase. J Biol Chem. 2003 Jul 18;278(29):26365-73. PMID:12721294 doi:10.1074/jbc.M304158200
- ↑ Yamada R, Yamahama Y, Sonobe H. Release of ecdysteroid-phosphates from egg yolk granules and their dephosphorylation during early embryonic development in silkworm, Bombyx mori. Zoolog Sci. 2005 Feb;22(2):187-98. PMID:15738639 doi:10.2108/zsj.22.187
- ↑ Chen Y, Jakoncic J, Wang J, Zheng X, Carpino N, Nassar N. Structural and functional characterization of the c-terminal domain of the ecdysteroid phosphate phosphatase from bombyx mori reveals a new enzymatic activity. Biochemistry. 2008 Nov 18;47(46):12135-45. Epub 2008 Oct 21. PMID:18937503 doi:10.1021/bi801318w
