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3deg is a 10 chain structure with sequence from Escherichia coli and Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
LEPA_ECOLI Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.[1][2]
Evolutionary Conservation
Checkto colour the structure by Evolutionary Conservation, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
↑ Qin Y, Polacek N, Vesper O, Staub E, Einfeldt E, Wilson DN, Nierhaus KH. The highly conserved LepA is a ribosomal elongation factor that back-translocates the ribosome. Cell. 2006 Nov 17;127(4):721-33. PMID:17110332 doi:http://dx.doi.org/10.1016/j.cell.2006.09.037
↑ Liu H, Pan D, Pech M, Cooperman BS. Interrupted catalysis: the EF4 (LepA) effect on back-translocation. J Mol Biol. 2010 Mar 5;396(4):1043-52. doi: 10.1016/j.jmb.2009.12.043. Epub 2010 , Jan 4. PMID:20045415 doi:http://dx.doi.org/10.1016/j.jmb.2009.12.043
