3df6

Publication Abstract from PubMed

Acidianus Filamentous Virus 1 (AFV1), isolated from acidic hot springs, is an enveloped lipid-containing archaeal filamentous virus with a linear double-stranded DNA genome. It infects Acidianus, which is a hyperthermostable archaea growing at 85 degrees C and acidic pHs, below pH 3. AFV1-99, a protein of 99 amino acids of unknown function, has homologues in the archaeal virus families Lipothrixviridae and Rudiviridae. We determined the crystal structure of AFV1-99 at 2.05 A resolution. AFV1-99 has a new fold, is hyperthermostable (up to 95 degrees C) and resists to extreme pH (between pH 0 and 11) and to the combination of high temperature (95 degrees C) and low pH (pH 0). It possesses characteristics of hyperthermostable proteins, such as a high content of charged residues.

The thermo- and acido-stable ORF-99 from the archaeal virus AFV1.,Goulet A, Spinelli S, Blangy S, van Tilbeurgh H, Leulliot N, Basta T, Prangishvili D, Cambillau C, Campanacci V Protein Sci. 2009 Jun;18(6):1316-20. PMID:19472363^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.