3fw6

From Proteopedia

Crystal structure of CelM2, a bifunctional glucanase-xylanase protein from a metagenome library

Structural highlights

3fw6 is a 1 chain structure with sequence from Uncultured bacterium. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.3Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A1E9A6_9BACT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Degradation of polysaccharides by cellulases and xylanases plays an important role in the carbon cycle, but only occurs in plant cell walls, a few bacteria and some animals. This process is also critical in processes such as biomass degradation and fuel production in the conversion cycles of cellulosic biomass. The enzyme CelM2 is bifunctional, because it is able to effectively hydrolyze barley glucan and xylan. Here, we show the crystal structure of the bifunctional enzyme CelM2, isolated from a metagenome library, and describe the sequence information and structure of its two domains. We believe that CelM2 is attractive as an industrial enzyme and that the structural results presented herein provide insights that are relevant to the genetic engineering of multifunctional enzymes.

Crystal structure of CelM2, a bifunctional glucanase-xylanase protein from a metagenome library.,Nam KH, Kim SJ, Hwang KY Biochem Biophys Res Commun. 2009 May 29;383(2):183-6. Epub 2009 Apr 2. PMID:19345197^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Nam KH, Kim SJ, Hwang KY. Crystal structure of CelM2, a bifunctional glucanase-xylanase protein from a metagenome library. Biochem Biophys Res Commun. 2009 May 29;383(2):183-6. Epub 2009 Apr 2. PMID:19345197 doi:10.1016/j.bbrc.2009.03.149