4d7k

Structural highlights

Function

ROSA_STRDJ Catalyzes the S-adenosyl methionine-dependent conversion of 8-amino-8-demethyl-D-riboflavin (AF) into 8-methylamino-8-demethyl-D-riboflavin (MAF) and roseoflavin (RoF), the last two steps in the biosynthesis of the antibiotic roseoflavin.^{[1] [2]}

References

1. ↑ Jankowitsch F, Kuhm C, Kellner R, Kalinowski J, Pelzer S, Macheroux P, Mack M. A novel N,N-8-amino-8-demethyl-D-riboflavin Dimethyltransferase (RosA) catalyzing the two terminal steps of roseoflavin biosynthesis in Streptomyces davawensis. J Biol Chem. 2011 Nov 4;286(44):38275-38285. doi: 10.1074/jbc.M111.292300. Epub, 2011 Sep 12. PMID:21911488 doi:http://dx.doi.org/10.1074/jbc.M111.292300
2. ↑ Tongsook C, Uhl MK, Jankowitsch F, Mack M, Gruber K, Macheroux P. Structural and kinetic studies on RosA, the enzyme catalysing the methylation of 8-demethyl-8-amino-D-riboflavin to the antibiotic roseoflavin. FEBS J. 2016 Feb 23. doi: 10.1111/febs.13690. PMID:26913589 doi:http://dx.doi.org/10.1111/febs.13690