4jo5

Publication Abstract from PubMed

The cellulosome of the cellulolytic bacterium Clostridium thermocellum has a structural multi-modular protein called CipA (cellulosome-integrating protein A) that includes nine enzyme-binding cohesin modules and a family 3 cellulose-binding module (CBM3a). In the CipA protein, the CBM3a module is located between the second and third cohesin modules and is connected to them via proline/threonine-rich linkers. The structure of CBM3a with portions of the C- and N-terminal flanking linker regions, CBM3a-L, has been determined to a resolution of 1.98 A. The structure is a beta-sandwich with a structural Ca(2+) ion. The structure is consistent with the previously determined CipA CBM structure; however, the structured linker regions provide a deeper insight into the overall cellulosome structure and assembly.

Structure of a family 3a carbohydrate-binding module from the cellulosomal scaffoldin CipA of Clostridium thermocellum with flanking linkers: implications for cellulosome structure.,Yaniv O, Morag E, Borovok I, Bayer EA, Lamed R, Frolow F, Shimon LJ Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Jul;69(Pt 7):733-7. doi:, 10.1107/S174430911301614X. Epub 2013 Jun 27. PMID:23832198^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.