4jp0

Structural highlights

Function

C35AB_BACTU Component of a binary insecticidal toxin active on western corn rootworm (WCR, Diabrotica virgifera subsp. virgifera Le Conte) and probably also on northern corn rootworm (D.barberi). Both proteins are required for maximal toxicity. The larval midgut epithelium is probably the primary target (PubMed:11433280, PubMed:11872461). This protein alone has no activity against southern corn rootworm (Diabrotica undecimpunctata howardi Barber), but it synergizes the toxic effect of its Cry34Ab1 partner (PubMed:12076012). The 2 proteins individually and together form ion channels; channels made in the presence of the 2 proteins have higher conductance (PubMed:15379574). Binds to WCR third instar midgut brush border membrane vesicles; binding improves over 10-fold in the presence of Cry34Ab1 (PubMed:23308139).^{[1] [2] [3] [4] [5]}

References

1. ↑ Moellenbeck DJ, Peters ML, Bing JW, Rouse JR, Higgins LS, Sims L, Nevshemal T, Marshall L, Ellis RT, Bystrak PG, Lang BA, Stewart JL, Kouba K, Sondag V, Gustafson V, Nour K, Xu D, Swenson J, Zhang J, Czapla T, Schwab G, Jayne S, Stockhoff BA, Narva K, Schnepf HE, Stelman SJ, Poutre C, Koziel M, Duck N. Insecticidal proteins from Bacillus thuringiensis protect corn from corn rootworms. Nat Biotechnol. 2001 Jul;19(7):668-72. doi: 10.1038/90282. PMID:11433280 doi:http://dx.doi.org/10.1038/90282
2. ↑ Ellis RT, Stockhoff BA, Stamp L, Schnepf HE, Schwab GE, Knuth M, Russell J, Cardineau GA, Narva KE. Novel Bacillus thuringiensis binary insecticidal crystal proteins active on western corn rootworm, Diabrotica virgifera virgifera LeConte. Appl Environ Microbiol. 2002 Mar;68(3):1137-45. PMID:11872461
3. ↑ Herman RA, Scherer PN, Young DL, Mihaliak CA, Meade T, Woodsworth AT, Stockhoff BA, Narva KE. Binary insecticidal crystal protein from Bacillus thuringiensis, strain PS149B1: effects of individual protein components and mixtures in laboratory bioassays. J Econ Entomol. 2002 Jun;95(3):635-9. doi: 10.1603/0022-0493-95.3.635. PMID:12076012 doi:http://dx.doi.org/10.1603/0022-0493-95.3.635
4. ↑ Masson L, Schwab G, Mazza A, Brousseau R, Potvin L, Schwartz JL. A novel Bacillus thuringiensis (PS149B1) containing a Cry34Ab1/Cry35Ab1 binary toxin specific for the western corn rootworm Diabrotica virgifera virgifera LeConte forms ion channels in lipid membranes. Biochemistry. 2004 Sep 28;43(38):12349-57. doi: 10.1021/bi048946z. PMID:15379574 doi:http://dx.doi.org/10.1021/bi048946z
5. ↑ Li H, Olson M, Lin G, Hey T, Tan SY, Narva KE. Bacillus thuringiensis Cry34Ab1/Cry35Ab1 interactions with western corn rootworm midgut membrane binding sites. PLoS One. 2013;8(1):e53079. doi: 10.1371/journal.pone.0053079. Epub 2013 Jan 4. PMID:23308139 doi:http://dx.doi.org/10.1371/journal.pone.0053079