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4jwi

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Crystal structure of the substrate binding domain of E.coli DnaK in complex with sheep Bac7(35-43)

Structural highlights

4jwi is a 4 chain structure with sequence from Escherichia coli K-12 and Ovis aries. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.9Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DNAK_ECOLI Plays an essential role in the initiation of phage lambda DNA replication, where it acts in an ATP-dependent fashion with the DnaJ protein to release lambda O and P proteins from the preprimosomal complex. DnaK is also involved in chromosomal DNA replication, possibly through an analogous interaction with the DnaA protein. Also participates actively in the response to hyperosmotic shock.[HAMAP-Rule:MF_00332]

Publication Abstract from PubMed

Bacterial resistance against common antibiotics is an increasing health problem. New pharmaceuticals for the treatment of infections caused by resistant pathogens are needed. Small proline-rich antimicrobial peptides (PrAMPs) from insects are known to bind intracellularly to the conventional substrate binding cleft of the E. coli Hsp70 chaperone DnaK. Furthermore, bactenecins from mammals, members of the cathelicidin family, also contain potential DnaK binding sites. Crystal structures of bovine and sheep Bac7 in complex with the DnaK substrate binding domain show that the peptides bind in the forward binding mode with a leucine positioned in the central hydrophobic pocket. In most structures, proline and arginine residues preceding leucine occupy the hydrophobic DnaK binding sites -1 and -2. Within bovine Bac7, four potential DnaK binding sites were identified.

Structural Identification of DnaK Binding Sites Within Bovine and Sheep Bactenecin Bac7.,Zahn M, Kieslich B, Berthold N, Knappe D, Hoffmann R, Strater N Protein Pept Lett. 2013 Oct 25. PMID:24164259^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zahn M, Kieslich B, Berthold N, Knappe D, Hoffmann R, Strater N. Structural Identification of DnaK Binding Sites Within Bovine and Sheep Bactenecin Bac7. Protein Pept Lett. 2013 Oct 25. PMID:24164259