4nso
From Proteopedia
Crystal structure of the effector-immunity protein complex
Structural highlights
FunctionVGRG3_VIBCH Part of the type VI secretion system specialized secretion system, which delivers several virulence factors in both prokaryotic and eukaryotic cells during infection (PubMed:23362380, PubMed:23341465). Forms the spike at the tip of the elongating tube formed by haemolysin co-regulated protein Hcp. Allows the delivery of the TseL antibacterial toxin to target cells where it exerts its toxicity (PubMed:23362380). Additionally, acts directly as an effector and targets the cell wall peptidoglycan layer of prey cells for degradation via its C-terminus (PubMed:23362380, PubMed:23341465). Toxicity is counteracted by a cognate immunity protein TsiV3 (PubMed:23362380, PubMed:24699653, PubMed:23341465).[1] [2] [3] Publication Abstract from PubMedThe bacterial type VI secretion system (T6SS) is used by donor cells to inject toxic effectors into receptor cells. The donor cells produce the corresponding immunity proteins to protect themselves against the effector proteins, thereby preventing their self-intoxication. Recently, the C-terminal domain of VgrG3 was identified as a T6SS effector. Information on the molecular mechanism of VgrG3 and its immunity protein TsaB has been lacking. Here, we determined the crystal structures of native TsaB and the VgrG3C-TsaB complex. VgrG3C adopts a canonical phage-T4-lysozyme-like fold. TsaB interacts with VgrG3C through molecular mimicry, and inserts into the VgrG3C pocket. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: VgrG3 and TsaBbind by x-ray crystallography (View interaction) TsaB and TsaBbind by x-ray crystallography (View interaction) VgrG3 and TsaBbind by cosedimentation in solution (View interaction) TsaB and TsaBbind by cosedimentation in solution (1,2) TsaBbinds to VgrG3 by surface plasmon resonance (1, 2, 3, 4, 5, 6, 7) VgrG3 and TsaBbind by molecular sieving (View interaction) TsaB and TsaBbind by molecular sieving (View interaction) VgrG3 and TsaBbind by x ray scattering (View interaction). Structural basis for recognition of the type VI spike protein VgrG3 by a cognate immunity protein.,Zhang J, Zhang H, Gao Z, Hu H, Dong C, Dong YH FEBS Lett. 2014 May 21;588(10):1891-8. doi: 10.1016/j.febslet.2014.04.016. Epub, 2014 Apr 18. PMID:24751834[4] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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