4qak

Structural highlights

Function

THPR_ECOLI Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester (PubMed:25239919). In vitro, can also ligate 5' and 3' half-tRNA molecules with 2',3'-cyclic phosphate and 5'-hydroxyl termini, respectively, to the product containing the 2'-5' phosphodiester linkage. This reaction does not require ATP and is reversible (PubMed:6347395, PubMed:8940112).^{[1] [2] [3]}

See Also

• RNA ligase

References

1. ↑ Remus BS, Jacewicz A, Shuman S. Structure and mechanism of E. coli RNA 2',3'-cyclic phosphodiesterase. RNA. 2014 Nov;20(11):1697-705. PMID:25239919 doi:10.1261/rna.046797.114
2. ↑ Greer CL, Javor B, Abelson J. RNA ligase in bacteria: formation of a 2',5' linkage by an E. coli extract. Cell. 1983 Jul;33(3):899-906. PMID:6347395 doi:10.1016/0092-8674(83)90032-6
3. ↑ Arn EA, Abelson JN. The 2'-5' RNA ligase of Escherichia coli. Purification, cloning, and genomic disruption. J Biol Chem. 1996 Dec 6;271(49):31145-53. PMID:8940112 doi:10.1074/jbc.271.49.31145