4rvz
From Proteopedia
Crystal structure of tRNA fluorescent labeling enzyme
Structural highlights
FunctionTIAS_ARCFU ATP-dependent agmatine transferase that catalyzes the formation of 2-agmatinylcytidine (agm2C) at the wobble position (C34) of tRNA(Ile2), converting the codon specificity from AUG to AUA.[1] Publication Abstract from PubMedAdvances in RNA research and RNA nanotechnology depend on the ability to manipulate and probe RNA with high precision through chemical approaches, both in vitro and in mammalian cells. However, covalent RNA labeling methods with scope and versatility comparable to those of current protein labeling strategies are underdeveloped. A method is reported for the site- and sequence-specific covalent labeling of RNAs in mammalian cells by using tRNAIle2 -agmatidine synthetase (Tias) and click chemistry. The crystal structure of Tias in complex with an azide-bearing agmatine analogue was solved to unravel the structural basis for Tias/substrate recognition. The unique RNA sequence specificity and plastic Tias/substrate recognition enable the site-specific transfer of azide/alkyne groups to an RNA molecule of interest in vitro and in mammalian cells. Subsequent click chemistry reactions facilitate the versatile labeling, functionalization, and visualization of target RNA. A Covalent Approach for Site-Specific RNA Labeling in Mammalian Cells.,Li F, Dong J, Hu X, Gong W, Li J, Shen J, Tian H, Wang J Angew Chem Int Ed Engl. 2015 Feb 18. doi: 10.1002/anie.201410433. PMID:25694369[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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