4v90

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Thermus thermophilus Ribosome

Structural highlights

4v90 is a 10 chain structure with sequence from Thermus thermophilus HB8. This structure supersedes the now removed PDB entries 4btd, 4cr1 and 4btc. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.95Å
Ligands:GCP, MG, ZN
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RS2_THET8 Spans the head-body hinge region of the 30S subunit. Is loosely associated with the 30S subunit.[HAMAP-Rule:MF_00291_B]

Publication Abstract from PubMed

In protein synthesis, elongation factor G (EF-G) facilitates movement of tRNA-mRNA by one codon, which is coupled to the ratchet-like rotation of the ribosome complex and is triggered by EF-G-mediated GTP hydrolysis. Here we report the structure of a pretranslocational ribosome bound to Thermus thermophilus EF-G trapped with a GTP analog. The positioning of the catalytic His87 into the active site coupled to hydrophobic-gate opening involves the 23S rRNA sarcin-ricin loop and domain III of EF-G and provides a structural basis for the GTPase activation of EF-G. Interactions of the hybrid peptidyl-site-exit-site tRNA with ribosomal elements, including the entire L1 stalk and proteins S13 and S19, shed light on how formation and stabilization of the hybrid tRNA is coupled to head swiveling and body rotation of the 30S as well as to closure of the L1 stalk.

Structure of EF-G-ribosome complex in a pretranslocation state.,Chen Y, Feng S, Kumar V, Ero R, Gao YG Nat Struct Mol Biol. 2013 Aug 4. doi: 10.1038/nsmb.2645. PMID:23912278[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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See Also

References

  1. Chen Y, Feng S, Kumar V, Ero R, Gao YG. Structure of EF-G-ribosome complex in a pretranslocation state. Nat Struct Mol Biol. 2013 Aug 4. doi: 10.1038/nsmb.2645. PMID:23912278 doi:10.1038/nsmb.2645

Contents


PDB ID 4v90

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