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From Proteopedia
Crystal structure of PfRh5, an essential P. falciparum ligand for invasion of human erythrocytes
Structural highlights
FunctionRH5_PLAF7 Essential for the invasion of host erythrocytes by blood stage merozoites (PubMed:25583518, PubMed:27374406, PubMed:25296023, PubMed:22080952, PubMed:18827878, PubMed:19000690, PubMed:31204103, PubMed:28186186, PubMed:28409866). By binding P113 at the surface of the merozoite and human BSG/basigin on the erythrocyte membrane, leads to the establishment of a tight junction between the merozoite and host erythrocyte membranes (PubMed:25583518, PubMed:27374406, PubMed:25296023, PubMed:22080952, PubMed:28186186). In addition, the interaction with BSG results in BSG dimerization which triggers an increase in intracellular Ca(2+) in the erythrocyte (PubMed:27374406, PubMed:28409866). This essential step leads to a rearrangement of the erythrocyte cytoskeleton required for the merozoite invasion (PubMed:28409866).[1] [2] [3] [4] [5] [6] [7] [8] [9] Publication Abstract from PubMedPlasmodium falciparum causes the most severe form of malaria in humans and is responsible for over 700,000 deaths annually. It is an obligate intracellular parasite and invades erythrocytes where it grows in a relatively protected niche. Invasion of erythrocytes is essential for parasite survival and this involves interplay of multiple protein-protein interactions. One of the most important interactions is binding of parasite invasion ligand families EBLs and PfRhs to host receptors on the surface of erythrocytes. PfRh5 is the only essential invasion ligand within the PfRh family and is an important vaccine candidate. PfRh5 binds the host receptor basigin. In this study, we have determined the crystal structure of PfRh5 using diffraction data to 2.18 A resolution. PfRh5 exhibits a novel fold, comprising nine mostly anti-parallel alpha-helices encasing an N-terminal beta-hairpin, with the overall shape being an elliptical disk. This is the first three-dimensional structure determined for the PfRh family of proteins. DOI: http://dx.doi.org/10.7554/eLife.04187.001 Crystal structure of PfRh5, an essential P. falciparum ligand for invasion of human erythrocytes.,Chen L, Xu Y, Healer J, Thompson JK, Smith BJ, Lawrence MC, Cowman AF Elife. 2014 Oct 8;3:e04187. doi: 10.7554/eLife.04187. PMID:25296023[10] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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