4yh5

Structural highlights

Function

LGK_LIPST Levoglucosan kinase that catalyzes the transfer of a phosphate group from ATP to levoglucosan (1,6-anhydro-beta-D-glucopyranose, LG) to yield glucose 6-phosphate in the presence of magnesium ion and ATP (Ref.1, PubMed:21719279, PubMed:26354439). In addition to the canonical kinase phosphotransfer reaction, the conversion requires cleavage of the 1,6-anhydro ring to allow ATP-dependent phosphorylation of the sugar O-6 atom (Ref.1, PubMed:21719279, PubMed:26354439).^{[1] [2] [3]}

References

1. ↑ Layton DS, Ajjarapu A, Choi DW, Jarboe LR. Engineering ethanologenic Escherichia coli for levoglucosan utilization. Bioresour Technol. 2011 Sep;102(17):8318-22. PMID:21719279 doi:10.1016/j.biortech.2011.06.011
2. ↑ Bacik JP, Klesmith JR, Whitehead TA, Jarboe LR, Unkefer CJ, Mark BL, Michalczyk R. Producing glucose-6-phosphate from cellulosic biomass: structural insights into levoglucosan bioconversion. J Biol Chem. 2015 Sep 9. pii: jbc.M115.674614. PMID:26354439 doi:http://dx.doi.org/10.1074/jbc.M115.674614
3. ↑ Layton DS, Ajjarapu A, Choi DW, Jarboe LR. Engineering ethanologenic Escherichia coli for levoglucosan utilization. Bioresour Technol. 2011 Sep;102(17):8318-22. PMID:21719279 doi:10.1016/j.biortech.2011.06.011