This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

4zkv

From Proteopedia

Jump to: navigation, search

Crystal structure of human histidine triad nucleotide-binding protein 1 (hHINT1) refined to 1.92A at P21 space group

Structural highlights

4zkv is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.92Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

HINT1_HUMAN Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2 (By similarity).

Publication Abstract from PubMed

Histidine triad nucleotide-binding protein 1 (HINT1) represents the most ancient and widespread branch in the histidine triad proteins superfamily. HINT1 plays an important role in various biological processes, and it has been found in many species. Here, we report the first structure (at a 2.34A resolution) of a complex of human HINT1 with a non-hydrolyzable analog of an Ap4A dinucleotide, containing bis-phosphorothioated glycerol mimicking a polyphosphate chain, obtained from a primitive monoclinic space group P21 crystal. In addition, the apo form of hHINT1 at the space group P21 refined to 1.92A is reported for comparative studies.

Crystallographic studies of the complex of human HINT1 protein with a non-hydrolyzable analog of ApA.,Dolot R, Kaczmarek R, Seda A, Krakowiak A, Baraniak J, Nawrot B Int J Biol Macromol. 2016 Feb 18;87:62-69. doi: 10.1016/j.ijbiomac.2016.02.047. PMID:26905466^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dolot R, Kaczmarek R, Seda A, Krakowiak A, Baraniak J, Nawrot B. Crystallographic studies of the complex of human HINT1 protein with a non-hydrolyzable analog of ApA. Int J Biol Macromol. 2016 Feb 18;87:62-69. doi: 10.1016/j.ijbiomac.2016.02.047. PMID:26905466 doi:http://dx.doi.org/10.1016/j.ijbiomac.2016.02.047