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Publication Abstract from PubMed

The ability to tune the light-absorption properties of chlorophylls by their protein environment is the key to the robustness and high efficiency of photosynthetic light-harvesting proteins. Unfortunately, the intricacy of the natural complexes makes it very difficult to identify and isolate specific protein-pigment interactions that underlie the spectral-tuning mechanisms. Herein we identify and demonstrate the tuning mechanism of chlorophyll spectra in type II water-soluble chlorophyll binding proteins from Brassicaceae (WSCPs). By comparing the molecular structures of two natural WSCPs we correlate a shift in the chlorophyll red absorption band with deformation of its tetrapyrrole macrocycle that is induced by changing the position of a nearby tryptophan residue. We show by a set of reciprocal point mutations that this change accounts for up to 2/3 of the observed spectral shift between the two natural variants.

Fine Tuning of Chlorophyll Spectra by Protein-Induced Ring Deformation.,Bednarczyk D, Dym O, Prabahar V, Peleg Y, Pike DH, Noy D Angew Chem Int Ed Engl. 2016 Apr 21. doi: 10.1002/anie.201512001. PMID:27098554^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.