5kxf
From Proteopedia
Crystal structure of the SPOC domain of the Arabidopsis flowering regulator FPA
Structural highlights
FunctionFPA_ARATH Plays a role in the regulation of flowering time in the autonomous flowering pathway by decreasing FLOWERING LOCUS C mRNA levels. Required for RNA-mediated chromatin silencing of a range of loci in the genome. Cotranscriptionally recognizes aberrant RNA and marks it for silencing. Controls alternative cleavage and polyadenylation on pre-mRNAs and antisense RNAs. Acts redundantly with FCA to prevent the expression of distally polyadenylated antisense RNAs at the FLC locus.[1] [2] [3] Publication Abstract from PubMedThe Arabidopsis protein FPA controls flowering time by regulating the alternative 3'-end processing of the FLOWERING LOCUS (FLC) antisense RNA. FPA belongs to the split ends (SPEN) family of proteins, which contain N-terminal RNA recognition motifs (RRMs) and a SPEN paralog and ortholog C-terminal (SPOC) domain. The SPOC domain is highly conserved among FPA homologs in plants, but the conservation with the domain in other SPEN proteins is much lower. We have determined the crystal structure of Arabidopsis thaliana FPA SPOC domain at 2.7 A resolution. The overall structure is similar to that of the SPOC domain in human SMRT/HDAC1 Associated Repressor Protein (SHARP), although there are also substantial conformational differences between them. Structural and sequence analyses identify a surface patch that is conserved among plant FPA homologs. Mutations of two residues in this surface patch did not disrupt FPA functions, suggesting that either the SPOC domain is not required for the role of FPA in regulating RNA 3'-end formation or the functions of the FPA SPOC domain cannot be disrupted by the combination of mutations, in contrast to observations with the SHARP SPOC domain. Crystal Structure of the SPOC Domain of the Arabidopsis Flowering Regulator FPA.,Zhang Y, Rataj K, Simpson GG, Tong L PLoS One. 2016 Aug 11;11(8):e0160694. doi: 10.1371/journal.pone.0160694., eCollection 2016. PMID:27513867[4] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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