5l7s
From Proteopedia
Crystal structure of RXLR effector PexRD54 from Phytophthora infestans
Structural highlights
FunctionRD54_PHYIT Effector that specifically binds host autophagy protein ATG8CL of the ATG8 family to stimulate autophagosome formation and subsequent autophagy rather than blocking autophagic flux (PubMed:26765567, PubMed:29932422). The pathogen remodels host-microbe interface by co-opting the host autophagy machinery which plays a key role in plant immunity (PubMed:29932422). PexRD54 competes with the autophagy cargo receptor Joka2 to deplete it out of ATG8CL complexes and interferes with Joka2's positive effect on pathogen defense (PubMed:26765567, PubMed:29932422).[1] [2] Publication Abstract from PubMedFilamentous plant pathogens deliver effector proteins to host cells to promote infection. The Phytophthora infestans RXLR-type effector PexRD54 binds potato ATG8 via its ATG8-family interacting motif (AIM) and perturbs host selective autophagy. However, the structural basis of this interaction remains unknown. Here we define the crystal structure of PexRD54, which comprises a modular architecture including five tandem repeat domains, with the AIM sequence presented at the disordered C-terminus. To determine the interface between PexRD54 and ATG8, we solved the crystal structure of potato ATG8CL in complex with a peptide comprising the effectors AIM sequence, and established a model of the full-length PexRD54/ATG8CL complex using small angle X-ray scattering. Structure-informed deletion of the PexRD54 tandem domains reveals retention of ATG8CL binding in vitro and in planta. This study offers new insights into structure/function relationships of oomycete RXLR effectors and how these proteins engage with host cell targets to promote disease. Structural basis of host Autophagy-related protein 8 (ATG8) binding by the Irish potato famine pathogen effector protein PexRD54.,Maqbool A, Hughes RK, Dagdas YF, Tregidgo N, Zess E, Belhaj K, Round A, Bozkurt TO, Kamoun S, Banfield MJ J Biol Chem. 2016 Jul 25. pii: jbc.M116.744995. PMID:27458016[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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