5ls8

Publication Abstract from PubMed

By using X-ray crystallography, we show that the complexes Lambda/Delta-[Ru(TAP)(2) (11-CN-dppz)](2+) (TAP=1,4,5,8-tetraazaphenanthrene, dppz=dipyridophenazine) bind DNA G-quadruplex in an enantiospecific manner that parallels the specificity of these complexes with duplex DNA. The Lambda complex crystallises with the normally parallel stranded d(TAGGGTTA) tetraplex to give the first such antiparallel strand assembly in which syn-guanosine is adjacent to the complex at the 5' end of the quadruplex core. SRCD measurements confirm that the same conformational switch occurs in solution. The Delta enantiomer, by contrast, is present in the structure but stacked at the ends of the assembly. In addition, we report the structure of Lambda-[Ru(phen)(2) (11-CN-dppz)](2+) bound to d(TCGGCGCCGA), a duplex-forming sequence, and use both structural models to provide insight into the motif-specific luminescence response of the isostructural phen analogue enantiomers.

Structural Studies Reveal Enantiospecific Recognition of a DNA G-Quadruplex by a Ruthenium Polypyridyl Complex.,McQuaid K, Abell H, Gurung SP, Allan DR, Winter G, Sorensen T, Cardin DJ, Brazier JA, Cardin CJ, Hall JP Angew Chem Int Ed Engl. 2019 Jul 15;58(29):9881-9885. doi: , 10.1002/anie.201814502. Epub 2019 Jun 12. PMID:30958918^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.