5nps

Publication Abstract from PubMed

O-GlcNAc transferase (OGT) is an essential glycosyltransferase that installs the O-GlcNAc post-translational modification on the nucleocytoplasmic proteome. We report the development of S-linked UDP-peptide conjugates as potent bisubstrate OGT inhibitors. These compounds were assembled in a modular fashion by photoinitiated thiol-ene conjugation of allyl-UDP and optimal acceptor peptides in which the acceptor serine was replaced with cysteine. The conjugate VTPVC(S-propyl-UDP)TA ( Ki = 1.3 muM) inhibits the OGT activity in HeLa cell lysates. Linear fusions of this conjugate with cell penetrating peptides were explored as prototypes of cell-penetrant OGT inhibitors. A crystal structure of human OGT with the inhibitor revealed mimicry of the interactions seen in the pseudo-Michaelis complex. Furthermore, a fluorophore-tagged derivative of the inhibitor works as a high affinity probe in a fluorescence polarimetry hOGT assay.

Thio-Linked UDP-Peptide Conjugates as O-GlcNAc Transferase Inhibitors.,Rafie K, Gorelik A, Trapannone R, Borodkin VS, van Aalten DMF Bioconjug Chem. 2018 May 10. doi: 10.1021/acs.bioconjchem.8b00194. PMID:29723473^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.