5x1e

Structural highlights

Function

ICMS_LEGPH Component of the Dot/Icm type IVB secretion system (T4BSS), which is used to inject bacterial effector proteins into eukaryotic host cells (PubMed:15661013, PubMed:18069892, PubMed:17040490, PubMed:22694730, PubMed:32513920). Part of a subcomplex which recruits effector proteins and delivers them to the core transmembrane subcomplex (PubMed:23028312, PubMed:32513920). The IcmS/IcmW protein complex plays an important role in protein translocation by interacting with multiple Dot/Icm effector proteins to facilitate their translocation into host cells (PubMed:15661013, PubMed:18069892). Interaction promotes conformational changes in the effector protein, which may facilitate display of a C-terminal translocation signal (PubMed:18069892). May maintain the substrates in a translocation competent form (PubMed:23028312). Required for intracellular growth in host cells, replicative phagosome formation and phagosome trafficking (PubMed:11115108). IcmS is required for IcmW stability (PubMed:15661013, PubMed:29203674).^{[1] [2] [3] [4] [5] [6] [7] [8]}

References

1. ↑ Coers J, Kagan JC, Matthews M, Nagai H, Zuckman DM, Roy CR. Identification of Icm protein complexes that play distinct roles in the biogenesis of an organelle permissive for Legionella pneumophila intracellular growth. Mol Microbiol. 2000 Nov;38(4):719-36. PMID:11115108 doi:10.1046/j.1365-2958.2000.02176.x
2. ↑ Ninio S, Zuckman-Cholon DM, Cambronne ED, Roy CR. The Legionella IcmS-IcmW protein complex is important for Dot/Icm-mediated protein translocation. Mol Microbiol. 2005 Feb;55(3):912-26. PMID:15661013 doi:10.1111/j.1365-2958.2004.04435.x
3. ↑ Vincent CD, Friedman JR, Jeong KC, Buford EC, Miller JL, Vogel JP. Identification of the core transmembrane complex of the Legionella Dot/Icm type IV secretion system. Mol Microbiol. 2006 Dec;62(5):1278-91. doi: 10.1111/j.1365-2958.2006.05446.x. , Epub 2006 Oct 16. PMID:17040490 doi:http://dx.doi.org/10.1111/j.1365-2958.2006.05446.x
4. ↑ Cambronne ED, Roy CR. The Legionella pneumophila IcmSW complex interacts with multiple Dot/Icm effectors to facilitate type IV translocation. PLoS Pathog. 2007 Dec;3(12):e188. PMID:18069892 doi:10.1371/journal.ppat.0030188
5. ↑ Vincent CD, Friedman JR, Jeong KC, Sutherland MC, Vogel JP. Identification of the DotL coupling protein subcomplex of the Legionella Dot/Icm type IV secretion system. Mol Microbiol. 2012 Jul;85(2):378-91. doi: 10.1111/j.1365-2958.2012.08118.x. Epub , 2012 Jun 14. PMID:22694730 doi:http://dx.doi.org/10.1111/j.1365-2958.2012.08118.x
6. ↑ Sutherland MC, Nguyen TL, Tseng V, Vogel JP. The Legionella IcmSW complex directly interacts with DotL to mediate translocation of adaptor-dependent substrates. PLoS Pathog. 2012 Sep;8(9):e1002910. doi: 10.1371/journal.ppat.1002910. Epub 2012 , Sep 13. PMID:23028312 doi:http://dx.doi.org/10.1371/journal.ppat.1002910
7. ↑ Xu J, Xu D, Wan M, Yin L, Wang X, Wu L, Liu Y, Liu X, Zhou Y, Zhu Y. Structural insights into the roles of the IcmS-IcmW complex in the type IVb secretion system of Legionella pneumophila. Proc Natl Acad Sci U S A. 2017 Dec 19;114(51):13543-13548. PMID:29203674 doi:10.1073/pnas.1706883115
8. ↑ Meir A, Mace K, Lukoyanova N, Chetrit D, Hospenthal MK, Redzej A, Roy C, Waksman G. Mechanism of effector capture and delivery by the type IV secretion system from Legionella pneumophila. Nat Commun. 2020 Jun 8;11(1):2864. doi: 10.1038/s41467-020-16681-z. PMID:32513920 doi:http://dx.doi.org/10.1038/s41467-020-16681-z