5x5i
From Proteopedia
The X-ray crystal structure of a TetR family transcription regulator RcdA involved in the regulation of biofilm formation in Escherichia coli
Structural highlights
FunctionRCDA_ECOLI Regulates the expression of a number of genes involved in biofilm formation and stress response. Target genes include six stress-response transcriptional regulators: csgD, which is a master regulator of biofilm formation, appY, sxy, ycgF, fimB and rcdA itself. This indicates that a large number of genes must be regulated indirectly via these transcriptional regulators. Acts by binding to the upstream region of its target genes.[1] Publication Abstract from PubMedRcdA is a regulator of curlin subunit gene D, the master regulator of biofilm formation in Escherichia coli. Here, we determined the X-ray structure of RcdA at 2.55 A resolution. RcdA consists of an N-terminal DNA-binding domain (DBD) containing a helix-turn-helix (HTH) motif and a C-terminal dimerization domain, and forms a homodimer in crystals. A computational docking model of the RcdA-DNA complex allowed prediction of the candidate residues responsible for DNA binding. Our structure-guided mutagenesis, in combination with gel shift assay, atomic force microscopic observation, and reporter assay, indicate that R32 in alpha2 of the HTH motif plays an essential role in the recognition and binding of target DNA while T46 in alpha3 influences the mode of oligomerization. These results provide insights into the DNA-binding mode of RcdA. A structural sketch of RcdA, a transcription factor controlling the master regulator of biofilm formation.,Sugino H, Usui T, Shimada T, Nakano M, Ogasawara H, Ishihama A, Hirata A FEBS Lett. 2017 Jul;591(13):2019-2031. doi: 10.1002/1873-3468.12713. Epub 2017, Jun 26. PMID:28608551[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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