5zc2

Structural highlights

Function

HPAHR_ACIBA Reductase component of a two-component system that supplies reduced FMN (FMNH2) to the oxygenase component to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-dihydroxyphenylacetate (3,4-DHPA). Catalyzes the reduction of free flavins (FMN, FAD and riboflavin) by NADH. Subsequently, the reduced flavins diffuse to the oxygenase component C2.^{[1] [2] [3]}

References

1. ↑ Chaiyen P, Suadee C, Wilairat P. A novel two-protein component flavoprotein hydroxylase. Eur J Biochem. 2001 Nov;268(21):5550-61. PMID:11683878
2. ↑ Sucharitakul J, Chaiyen P, Entsch B, Ballou DP. The reductase of p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii requires p-hydroxyphenylacetate for effective catalysis. Biochemistry. 2005 Aug 2;44(30):10434-42. PMID:16042421 doi:http://dx.doi.org/10.1021/bi050615e
3. ↑ Sucharitakul J, Phongsak T, Entsch B, Svasti J, Chaiyen P, Ballou DP. Kinetics of a two-component p-hydroxyphenylacetate hydroxylase explain how reduced flavin is transferred from the reductase to the oxygenase. Biochemistry. 2007 Jul 24;46(29):8611-23. Epub 2007 Jun 27. PMID:17595116 doi:http://dx.doi.org/10.1021/bi7006614