| Structural highlights
Function
KET3E_ARTGO Catalyzes the reversible C-3 epimerization of several ketoses. Shows the highest enzymatic activity for the epimerization of L-ribulose to L-xylulose. Is also able to convert D-allulose (also known as D-psicose) to D-fructose and, to a lesser extent, L-tagatose to L-sorbose, D-ribulose to D-xylulose, L-allulose to L-fructose and D-tagatose to D-sorbose.[1] [2]
Publication Abstract from PubMed
The X-ray structure of ketose 3-epimerase from Arthrobacter globiformis M30, which was previously reported to be a D-allulose 3-epimerase (AgD-AE), was determined at 1.96 A resolution. The crystal belonged to the hexagonal space group P6522, with unit-cell parameters a = b = 103.98, c = 256.53 A. The structure was solved by molecular replacement using the structure of Mesorhizobium loti L-ribulose 3-epimerase (MlL-RE), which has 41% sequence identity, as a search model. A hexagonal crystal contained two molecules in the asymmetric unit, and AgD-AE formed a homotetramer with twofold symmetry. The overall structure of AgD-AE was more similar to that of MlL-RE than to the known structures of D-psicose (alternative name D-allulose) 3-epimerases (D-PEs or D-AEs), although AgD-AE and MlL-RE have different substrate specificities. Both AgD-AE and MlL-RE have long helices in the C-terminal region that would contribute to the stability of the homotetramer. AgD-AE showed higher enzymatic activity for L-ribulose than D-allulose; however, AgD-AE is stable and is a unique useful enzyme for the production of D-allulose from D-fructose.
X-ray structure of Arthrobacter globiformis M30 ketose 3-epimerase for the production of D-allulose from D-fructose.,Yoshida H, Yoshihara A, Gullapalli PK, Ohtani K, Akimitsu K, Izumori K, Kamitori S Acta Crystallogr F Struct Biol Commun. 2018 Oct 1;74(Pt 10):669-676. doi:, 10.1107/S2053230X18011706. Epub 2018 Sep 21. PMID:30279320[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Yoshihara A, Kozakai T, Shintani T, Matsutani R, Ohtani K, Iida T, Akimitsu K, Izumori K, Gullapalli PK. Purification and characterization of d-allulose 3-epimerase derived from Arthrobacter globiformis M30, a GRAS microorganism. J Biosci Bioeng. 2017 Feb;123(2):170-176. PMID:27713017 doi:10.1016/j.jbiosc.2016.09.004
- ↑ Yoshida H, Yoshihara A, Gullapalli PK, Ohtani K, Akimitsu K, Izumori K, Kamitori S. X-ray structure of Arthrobacter globiformis M30 ketose 3-epimerase for the production of D-allulose from D-fructose. Acta Crystallogr F Struct Biol Commun. 2018 Oct 1;74(Pt 10):669-676. doi:, 10.1107/S2053230X18011706. Epub 2018 Sep 21. PMID:30279320 doi:http://dx.doi.org/10.1107/S2053230X18011706
- ↑ Yoshida H, Yoshihara A, Gullapalli PK, Ohtani K, Akimitsu K, Izumori K, Kamitori S. X-ray structure of Arthrobacter globiformis M30 ketose 3-epimerase for the production of D-allulose from D-fructose. Acta Crystallogr F Struct Biol Commun. 2018 Oct 1;74(Pt 10):669-676. doi:, 10.1107/S2053230X18011706. Epub 2018 Sep 21. PMID:30279320 doi:http://dx.doi.org/10.1107/S2053230X18011706
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