6c5y

Publication Abstract from PubMed

With the recent developments in microcrystal handling, synchrotron microdiffraction beamline instrumentation and data analysis, microcrystal crystallo-graphy with crystal sizes of less than 10 microm is appealing at synchrotrons. However, challenges remain in sample manipulation and data assembly for robust microcrystal synchrotron crystallography. Here, the development of micro-sized polyimide well-mounts for the manipulation of microcrystals of a few micrometres in size and the implementation of a robust data-analysis method for the assembly of rotational microdiffraction data sets from many microcrystals are described. The method demonstrates that microcrystals may be routinely utilized for the acquisition and assembly of complete data sets from synchrotron microdiffraction beamlines.

Sample manipulation and data assembly for robust microcrystal synchrotron crystallography.,Guo G, Fuchs MR, Shi W, Skinner J, Berman E, Ogata CM, Hendrickson WA, McSweeney S, Liu Q IUCrJ. 2018 Apr 19;5(Pt 3):238-246. doi: 10.1107/S2052252518005389. eCollection, 2018 May 1. PMID:29755741^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.