6dte

From Proteopedia

GlcNAc-inspired cyclophellitol bound to NagZ

Structural highlights

6dte is a 2 chain structure with sequence from Burkholderia cenocepacia. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.929Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A125HFC0_9BURK Plays a role in peptidoglycan recycling by cleaving the terminal beta-1,4-linked N-acetylglucosamine (GlcNAc) from peptide-linked peptidoglycan fragments, giving rise to free GlcNAc, anhydro-N-acetylmuramic acid and anhydro-N-acetylmuramic acid-linked peptides.[HAMAP-Rule:MF_00364][SAAS:SAAS00634279]

Publication Abstract from PubMed

The development of a potent mechanism-based inactivator of NagZ, an enzyme critical to the production of inducible AmpC beta-lactamase in Gram-negative bacteria, is presented. This inactivator significantly reduces MIC values for important beta-lactams against a clinically relevant strain of Pseudomonas aeruginosa.

A mechanism-based GlcNAc-inspired cyclophellitol inactivator of the peptidoglycan recycling enzyme NagZ reverses resistance to beta-lactams in Pseudomonas aeruginosa.,Ho LA, Winogrodzki JL, Debowski AW, Madden Z, Vocadlo DJ, Mark BL, Stubbs KA Chem Commun (Camb). 2018 Sep 25;54(75):10630-10633. doi: 10.1039/c8cc05281f. Epub, 2018 Sep 4. PMID:30178799^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ho LA, Winogrodzki JL, Debowski AW, Madden Z, Vocadlo DJ, Mark BL, Stubbs KA. A mechanism-based GlcNAc-inspired cyclophellitol inactivator of the peptidoglycan recycling enzyme NagZ reverses resistance to beta-lactams in Pseudomonas aeruginosa. Chem Commun (Camb). 2018 Sep 25;54(75):10630-10633. doi: 10.1039/c8cc05281f. Epub, 2018 Sep 4. PMID:30178799 doi:http://dx.doi.org/10.1039/c8cc05281f