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Publication Abstract from PubMed

Resonance assignment and structural characterization of pharmacologically-relevant proteins promises to improve their understanding and safety by rational design. However, the PEG coating that is used to evade the immune system also causes these molecules to "evade" the standard structural biology methodologies. We here demonstrate that it is possible to obtain the resonance assignment and a reliable structural model of large PEGylated proteins through an integrated approach encompassing NMR and X-ray crystallography.

Characterization of PEGylated asparaginase: new opportunities from NMR analysis of large pegylated therapeutics.,Cerofolini L, Giuntini S, Carlon A, Ravera E, Calderone V, Fragai M, Parigi G, Luchinat C Chemistry. 2018 Nov 21. doi: 10.1002/chem.201804488. PMID:30462348^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.