Structural highlights
Function
[DEGLY_PYRHO] Deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins and reactive carbonyl groups of glyoxals. Thus, functions as a protein deglycase that repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of advanced glycation endproducts (AGE) that cause irreversible damage (By similarity). Also displays proteolytic activity (PubMed:11114201).[UniProtKB:Q51732][1]
Publication Abstract from PubMed
Obtaining crystals and solving the phase problem remain major hurdles encountered by bio-crystallographers in their race to obtain new high-quality structures. Both issues can be overcome by the crystallophore, Tb-Xo4, a lanthanide-based molecular complex with unique nucleating and phasing properties. This article presents examples of new crystallization conditions induced by the presence of Tb-Xo4. These new crystalline forms bypass crystal defects often encountered by crystallographers, such as low-resolution diffracting samples or crystals with twinning. Thanks to Tb-Xo4's high phasing power, the structure determination process is greatly facilitated and can be extended to serial crystallography approaches.
Protein crystal structure determination with the crystallophore, a nucleating and phasing agent.,Engilberge S, Wagner T, Santoni G, Breyton C, Shima S, Franzetti B, Riobe F, Maury O, Girard E J Appl Crystallogr. 2019 Jun 28;52(Pt 4):722-731. doi: 10.1107/S1600576719006381., eCollection 2019 Aug 1. PMID:31396026[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Du X, Choi IG, Kim R, Wang W, Jancarik J, Yokota H, Kim SH. Crystal structure of an intracellular protease from Pyrococcus horikoshii at 2-A resolution. Proc Natl Acad Sci U S A. 2000 Dec 19;97(26):14079-84. PMID:11114201 doi:10.1073/pnas.260503597
- ↑ Engilberge S, Wagner T, Santoni G, Breyton C, Shima S, Franzetti B, Riobe F, Maury O, Girard E. Protein crystal structure determination with the crystallophore, a nucleating and phasing agent. J Appl Crystallogr. 2019 Jun 28;52(Pt 4):722-731. doi: 10.1107/S1600576719006381., eCollection 2019 Aug 1. PMID:31396026 doi:http://dx.doi.org/10.1107/S1600576719006381
