Structural highlights
Function
NDMB_PSEPU Involved in the caffeine degradation, which is the essential first step for assimilating the carbon and nitrogen in caffeine. Catalyzes the N3-demethylation of theobromine to produce 7-methylxanthine and formaldehyde. Also catalyzes the N3-demethylation of 3-methylxanthine, caffeine, and theophylline to xanthine, paraxanthine, and 1-methylxanthine, respectively. NADH is the preferred substrate.[1] [2]
References
- ↑ Summers RM, Louie TM, Yu CL, Subramanian M. Characterization of a broad-specificity non-haem iron N-demethylase from Pseudomonas putida CBB5 capable of utilizing several purine alkaloids as sole carbon and nitrogen source. Microbiology. 2011 Feb;157(Pt 2):583-92. doi: 10.1099/mic.0.043612-0. Epub 2010, Oct 21. PMID:20966097 doi:http://dx.doi.org/10.1099/mic.0.043612-0
- ↑ Summers RM, Louie TM, Yu CL, Gakhar L, Louie KC, Subramanian M. Novel, highly specific N-demethylases enable bacteria to live on caffeine and related purine alkaloids. J Bacteriol. 2012 Apr;194(8):2041-9. doi: 10.1128/JB.06637-11. Epub 2012 Feb 10. PMID:22328667 doi:http://dx.doi.org/10.1128/JB.06637-11
