6igi
From Proteopedia
Crystal structure of FT condition 2
Structural highlights
FunctionFT_ARATH Probable component of the mobile flower-promoting signal (floral stimulus or florigen). Promotes the transition from vegetative growth to flowering. Required for 'SEPALLATA3' (SEP3) and 'FRUITFULL' (FUL) accumulation in mature rosette leaves. Seems to acts in parallel with 'LEAFY' to induce flowering by regulating 'APETALA1'. May play a role in both the autonomous and the long-day flowering pathways.[1] [2] [3] Publication Abstract from PubMedArabidopsis FLOWERING LOCUS T (FT) is a pivotal component of florigen, a long-range mobile flowering signal. Here, we determined the 1.0 A-resolution crystal structure of FT, a significantly higher-resolution crystal structure of FT than previously reported one (2.6 A). The present crystallographic studies revealed 4 alternative configurations with the precise location of the surrounding water molecules. Using this structural data, computational docking simulation predicted the putative binding sites for phosphatidylcholine (PC), an endogenous ligand that interacts with FT to modulate flowering time. In vitro reconstitution of the lipid-protein interaction showed that mutations at two of the predicted sites significantly compromised the lipid binding ability of FT. In planta, one of the mutant FT proteins significantly affected FT function in flowering, emphasizing the involvement of PC binding in modulating FT function. Our structural, biochemical, and transgenic analyses reveal the molecular mechanism of PC binding in FT-mediated flowering time control. High-Resolution Crystal Structure of Arabidopsis FLOWERING LOCUS T Illuminates Its Phospholipid-Binding Site in Flowering.,Nakamura Y, Lin YC, Watanabe S, Liu YC, Katsuyama K, Kanehara K, Inaba K iScience. 2019 Nov 22;21:577-586. doi: 10.1016/j.isci.2019.10.045. Epub 2019 Oct , 26. PMID:31726375[4] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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