6ixj
From Proteopedia
The crystal structure of sulfoacetaldehyde reductase from Klebsiella oxytoca
Structural highlights
FunctionISFD_KLEOX Catalyzes the formation of isethionate from 2-sulfoacetaldehyde in the deaminative pathway of taurine. The enzyme is specific for NADPH; NADH is not a substrate.[1] Publication Abstract from PubMedSulfoacetaldehyde reductase (IsfD) is a member of the short-chain dehydrogenase/reductase (SDR) family, involved in nitrogen assimilation from aminoethylsulfonate (taurine) in certain environmental and human commensal bacteria. IsfD catalyzes the reversible NADPH-dependent reduction of sulfoacetaladehyde, which is generated by transamination of taurine, forming hydroxyethylsulfonate (isethionate) as a waste product. In this study, the crystal structure of Klebsiella oxytoca IsfD in a ternary complex with NADPH and isethionate, was solved at 2.8 A, revealing residues important for substrate binding. IsfD forms a homotetramer in both crystal and solution states, with the C-terminal tail of each subunit interacting with the C-terminal tail of the diagonally opposite subunit, forming an anti-parallel beta sheet that constitutes part of the substrate binding site. The sulfonate group of isethionate is stabilized by a hydrogen bond network formed by the residues Y148, R195, Q244 and a water molecule. In addition, F249 from the diagonal subunit restrains the conformation of Y148 to further stabilize the orientation of the sulfonate group. Mutation of any of these four residues into alanine resulted in a complete loss of catalytic activity for isethionate oxidation. Biochemical investigations of the substrate scope of IsfD, and bioinformatics analysis of IsfD homologs, suggest that IsfD is related to the promiscuous 3-hydroxyacid dehydrogenases with diverse metabolic functions. Biochemical and structural investigation of sulfoacetaldehyde reductase from Klebsiella oxytoca.,Zhou Y, Wei Y, Lin L, Xu T, Ang EL, Zhao H, Yuchi Z, Zhang Y Biochem J. 2019 Feb 4. pii: BCJ20190005. doi: 10.1042/BCJ20190005. PMID:30718306[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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Categories: Klebsiella oxytoca | Large Structures | Lin L | Xu T | Yuchi Z | Zhang Y | Zhou Y