6j43

Publication Abstract from PubMed

Microcrystal delivery methods are pivotal in the use of serial femtosecond crystallography (SFX) to resolve the macromolecular structures of proteins. Here, the development of a novel technique and instruments for efficiently delivering microcrystals for SFX are presented. The new method, which relies on a one-dimensional fixed-target system that includes a microcrystal container, consumes an extremely low amount of sample compared with conventional two-dimensional fixed-target techniques at ambient temperature. This novel system can deliver soluble microcrystals without highly viscous carrier media and, moreover, can be used as a microcrystal growth device for SFX. Diffraction data collection utilizing this advanced technique along with a real-time visual servo scan system has been successfully demonstrated for the structure determination of proteinase K microcrystals at 1.85 A resolution.

Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography.,Lee D, Park S, Lee K, Kim J, Park G, Nam KH, Baek S, Chung WK, Lee JL, Cho Y, Park J J Appl Crystallogr. 2020 Mar 25;53(Pt 2):477-485. doi: 10.1107/S1600576720002423., eCollection 2020 Apr 1. PMID:32280322^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.