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Publication Abstract from PubMed

G-quadruplex DNA show structural polymorphism, leading to challenges in the use of selective recognition probes for the accurate detection of G-quadruplexes in vivo. Herein, we present a tripodal cationic fluorescent probe, NBTE, which showed distinguishable fluorescence lifetime responses between G-quadruplexes and other DNA topologies, and fluorescence quantum yield (Phif ) enhancement upon G-quadruplex binding. We determined two NBTE-G-quadruplex complex structures with high Phif values by NMR spectroscopy. The structures indicated NBTE interacted with G-quadruplexes using three arms through pi-pi stacking, differing from that with duplex DNA using two arms, which rationalized the higher Phif values and lifetime response of NBTE upon G-quadruplex binding. Based on photon counts of FLIM, we detected the percentage of G-quadruplex DNA in live cells with NBTE and found G-quadruplex DNA content in cancer cells is 4-fold that in normal cells, suggesting the potential applications of this probe in cancer cell detection.

Quantitative Detection of G-Quadruplex DNA in Live Cells Based on Photon Counts and Complex Structure Discrimination.,Liu LY, Liu W, Wang KN, Zhu BC, Xia XY, Ji LN, Mao ZW Angew Chem Int Ed Engl. 2020 Jun 8;59(24):9719-9726. doi: 10.1002/anie.202002422., Epub 2020 Apr 1. PMID:32173994^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.