6kln

From Proteopedia

F-actin of cardiac thin filament in high-calcium state

6kln, resolution 3.40Å

Structural highlights

6kln is a 4 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.4Å
Ligands:
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ACTS_MOUSE Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Publication Abstract from PubMed

Troponin is an essential component of striated muscle and it regulates the sliding of actomyosin system in a calcium-dependent manner. Despite its importance, the structure of troponin has been elusive due to its high structural heterogeneity. In this study, we analyzed the 3D structures of murine cardiac thin filaments using a cryo-electron microscope equipped with a Volta phase plate (VPP). Contrast enhancement by a VPP enabled us to reconstruct the entire repeat of the thin filament. We determined the orientation of troponin relative to F-actin and tropomyosin, and characterized the interactions between troponin and tropomyosin. This study provides a structural basis for understanding the molecular mechanism of actomyosin system.

Cryo-EM structures of cardiac thin filaments reveal the 3D architecture of troponin.,Oda T, Yanagisawa H, Wakabayashi T J Struct Biol. 2020 Jan 16:107450. doi: 10.1016/j.jsb.2020.107450. PMID:31954841^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Oda T, Yanagisawa H, Wakabayashi T. Cryo-EM structures of cardiac thin filaments reveal the 3D architecture of troponin. J Struct Biol. 2020 Jan 16:107450. doi: 10.1016/j.jsb.2020.107450. PMID:31954841 doi:http://dx.doi.org/10.1016/j.jsb.2020.107450