6lir

From Proteopedia

crystal structure of chicken TCR for 2.0

Structural highlights

6lir is a 4 chain structure with sequence from Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.091Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

In this work, chicken HPAIV H5N1 epitope-specific TCRalphabeta (ch-TCRalphabeta) was isolated and its structure was determined. The Calpha domain of ch-TCRalphabeta does not exhibit the typical structure of human TCRalphabeta, and the DE loop extends outward, resulting in close proximity between the Calpha domain of ch-TCRalphabeta and CD3epsilondelta/gamma. The FG loop of the Cbeta domain of ch-TCRalphabeta is shorter. The changes in the C domains of ch-TCRalphabeta and the difference in chicken CD3epsilondelta/gamma confirm that the complexes formed by TCRalphabeta and CD3epsilondelta/gamma differ from those in humans. In the chicken complex, a positively charged cleft is formed between the two CDR3 loops that might accommodate the acidic side chains of the chicken pMHC-I-bound HPAIV epitope intermediate portion oriented toward ch-TCRalphabeta. This is the first reported structure of chicken TCRalphabeta, and it provides a structural model of the ancestral TCR system in the immune synapses between T cells and antigen-presenting cells in lower vertebrates.

Structural and Biophysical Insights into the TCRalphabeta Complex in Chickens.,Zhang L, Liu Y, Meng G, Liang R, Zhang B, Xia C iScience. 2020 Nov 20;23(12):101828. doi: 10.1016/j.isci.2020.101828. eCollection , 2020 Dec 18. PMID:33305184^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhang L, Liu Y, Meng G, Liang R, Zhang B, Xia C. Structural and Biophysical Insights into the TCRαβ Complex in Chickens. iScience. 2020 Nov 20;23(12):101828. PMID:33305184 doi:10.1016/j.isci.2020.101828