6lul

Structural highlights

Function

RCF2_YEAST Assembly factor that plays a role in the assembly of the respiratory chain supercomplexes (SCs) composed of ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV). May be required for late-stage assembly of the COX12 and COX13 subunits (PubMed:22342701, PubMed:22310663). Required for the generation and maintenance of a normal proton motive force (PMF) across the inner mitochondrial membrane (IMM) by preventing proton leakage through an inactive population of CIV that accumulates when RCF1 and/or RCF2 proteins are absent (PubMed:30683696, PubMed:31591265).^{[1] [2] [3] [4]}

References

1. ↑ Strogolova V, Furness A, Robb-McGrath M, Garlich J, Stuart RA. Rcf1 and Rcf2, members of the hypoxia-induced gene 1 protein family, are critical components of the mitochondrial cytochrome bc1-cytochrome c oxidase supercomplex. Mol Cell Biol. 2012 Apr;32(8):1363-73. doi: 10.1128/MCB.06369-11. Epub 2012 Feb, 6. PMID:22310663 doi:http://dx.doi.org/10.1128/MCB.06369-11
2. ↑ Vukotic M, Oeljeklaus S, Wiese S, Vogtle FN, Meisinger C, Meyer HE, Zieseniss A, Katschinski DM, Jans DC, Jakobs S, Warscheid B, Rehling P, Deckers M. Rcf1 mediates cytochrome oxidase assembly and respirasome formation, revealing heterogeneity of the enzyme complex. Cell Metab. 2012 Mar 7;15(3):336-47. doi: 10.1016/j.cmet.2012.01.016. Epub 2012, Feb 16. PMID:22342701 doi:http://dx.doi.org/10.1016/j.cmet.2012.01.016
3. ↑ Strogolova V, Hoang NH, Hosler J, Stuart RA. The yeast mitochondrial proteins Rcf1 and Rcf2 support the enzymology of the cytochrome c oxidase complex and generation of the proton motive force. J Biol Chem. 2019 Mar 29;294(13):4867-4877. doi: 10.1074/jbc.RA118.006888. Epub, 2019 Jan 25. PMID:30683696 doi:http://dx.doi.org/10.1074/jbc.RA118.006888
4. ↑ Hoang NH, Strogolova V, Mosley JJ, Stuart RA, Hosler J. Hypoxia-inducible gene domain 1 proteins in yeast mitochondria protect against proton leak through complex IV. J Biol Chem. 2019 Nov 15;294(46):17669-17677. doi: 10.1074/jbc.RA119.010317. Epub, 2019 Oct 7. PMID:31591265 doi:http://dx.doi.org/10.1074/jbc.RA119.010317