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Publication Abstract from PubMed

Receptor-mediated perception of surface-exposed carbohydrates like lipo- and exo-polysaccharides (EPS) is important for non-self recognition and responses to microbial associated molecular patterns in mammals and plants. In legumes, EPS are monitored and can either block or promote symbiosis with rhizobia depending on their molecular composition. To establish a deeper understanding of receptors involved in EPS recognition, we determined the structure of the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain. EPR3 forms a compact structure built of three putative carbohydrate-binding modules (M1, M2 and LysM3). M1 and M2 have unique betaalphabetabeta and betaalphabeta folds that have not previously been observed in carbohydrate binding proteins, while LysM3 has a canonical betaalphaalphabeta fold. We demonstrate that this configuration is a structural signature for a ubiquitous class of receptors in the plant kingdom. We show that EPR3 is promiscuous, suggesting that plants can monitor complex microbial communities though this class of receptors.

Structural signatures in EPR3 define a unique class of plant carbohydrate receptors.,Wong JEMM, Gysel K, Birkefeldt TG, Vinther M, Muszynski A, Azadi P, Laursen NS, Sullivan JT, Ronson CW, Stougaard J, Andersen KR Nat Commun. 2020 Jul 30;11(1):3797. doi: 10.1038/s41467-020-17568-9. PMID:32732998^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.