| Structural highlights
Function
SBIR1_ARATH Dual specificity kinase acting on both serine/threonine- and tyrosine-containing substrates. Acting as a counterplayer of BIR1, promotes the activation of plant defense and cell death (PubMed:19616764). Component of the RLP23-SOBIR1-BAK1 complex that mediates NLP-triggered immunity (PubMed:27251392). Functions as an inhibitor/regulator of abscission, probably by regulating membrane trafficking during abscission (PubMed:20081191).[1] [2] [3]
Publication Abstract from PubMed
Plant-unique membrane receptor kinases with leucine-rich repeat (LRR) extracellular domains are key regulators of development and immune responses. Here, the 1.55 A resolution crystal structure of the immune receptor kinase SOBIR1 from Arabidopsis is presented. The ectodomain structure reveals the presence of five LRRs sandwiched between noncanonical capping domains. The disulfide-bond-stabilized N-terminal cap harbours an unusual beta-hairpin structure. The C-terminal cap features a highly positively charged linear motif which was found to be largely disordered in this structure. Size-exclusion chromatography and right-angle light-scattering experiments suggest that SOBIR1 is a monomer in solution. The protruding beta-hairpin, a set of highly conserved basic residues at the inner surface of the SOBIR LRR domain and the presence of a genetic missense allele in LRR2 together suggest that the SOBIR1 ectodomain may mediate protein-protein interaction in plant immune signalling.
Crystal structure of the leucine-rich repeat ectodomain of the plant immune receptor kinase SOBIR1.,Hohmann U, Hothorn M Acta Crystallogr D Struct Biol. 2019 May 1;75(Pt 5):488-497. doi:, 10.1107/S2059798319005291. Epub 2019 Apr 29. PMID:31063151[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gao M, Wang X, Wang D, Xu F, Ding X, Zhang Z, Bi D, Cheng YT, Chen S, Li X, Zhang Y. Regulation of cell death and innate immunity by two receptor-like kinases in Arabidopsis. Cell Host Microbe. 2009 Jul 23;6(1):34-44. doi: 10.1016/j.chom.2009.05.019. PMID:19616764 doi:http://dx.doi.org/10.1016/j.chom.2009.05.019
- ↑ Leslie ME, Lewis MW, Youn JY, Daniels MJ, Liljegren SJ. The EVERSHED receptor-like kinase modulates floral organ shedding in Arabidopsis. Development. 2010 Feb;137(3):467-76. doi: 10.1242/dev.041335. PMID:20081191 doi:http://dx.doi.org/10.1242/dev.041335
- ↑ Albert I, Bohm H, Albert M, Feiler CE, Imkampe J, Wallmeroth N, Brancato C, Raaymakers TM, Oome S, Zhang H, Krol E, Grefen C, Gust AA, Chai J, Hedrich R, Van den Ackerveken G, Nurnberger T. An RLP23-SOBIR1-BAK1 complex mediates NLP-triggered immunity. Nat Plants. 2015 Oct 5;1:15140. doi: 10.1038/nplants.2015.140. PMID:27251392 doi:http://dx.doi.org/10.1038/nplants.2015.140
- ↑ Hohmann U, Hothorn M. Crystal structure of the leucine-rich repeat ectodomain of the plant immune receptor kinase SOBIR1. Acta Crystallogr D Struct Biol. 2019 May 1;75(Pt 5):488-497. doi:, 10.1107/S2059798319005291. Epub 2019 Apr 29. PMID:31063151 doi:http://dx.doi.org/10.1107/S2059798319005291
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