Structural highlights
Function
RPA12_YEAST DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Involved in transcriptional termination. Involved in recruitment of RPA49 to Pol I.[1]
Publication Abstract from PubMed
RNA polymerase I (Pol I) assembles with core factor (CF) and Rrn3 on the rDNA core promoter for transcription initiation. Here, we report cryo-EM structures of closed, intermediate and open Pol I initiation complexes from 2.7 to 3.7 A resolution to visualize Pol I promoter melting and to structurally and biochemically characterize the recognition mechanism of Pol I promoter DNA. In the closed complex, double-stranded DNA runs outside the DNA-binding cleft. Rotation of CF and upstream DNA with respect to Pol I and Rrn3 results in the spontaneous loading and opening of the promoter followed by cleft closure and positioning of the Pol I A49 tandem winged helix domain (tWH) onto DNA. Conformational rearrangement of A49 tWH leads to a clash with Rrn3 to initiate complex disassembly and promoter escape. Comprehensive insight into the Pol I transcription initiation cycle allows comparisons with promoter opening by Pol II and Pol III.
Molecular insight into RNA polymerase I promoter recognition and promoter melting.,Sadian Y, Baudin F, Tafur L, Murciano B, Wetzel R, Weis F, Muller CW Nat Commun. 2019 Dec 5;10(1):5543. doi: 10.1038/s41467-019-13510-w. PMID:31804486[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Prescott EM, Osheim YN, Jones HS, Alen CM, Roan JG, Reeder RH, Beyer AL, Proudfoot NJ. Transcriptional termination by RNA polymerase I requires the small subunit Rpa12p. Proc Natl Acad Sci U S A. 2004 Apr 20;101(16):6068-73. Epub 2004 Apr 8. PMID:15073335 doi:http://dx.doi.org/10.1073/pnas.0401393101
- ↑ Sadian Y, Baudin F, Tafur L, Murciano B, Wetzel R, Weis F, Muller CW. Molecular insight into RNA polymerase I promoter recognition and promoter melting. Nat Commun. 2019 Dec 5;10(1):5543. doi: 10.1038/s41467-019-13510-w. PMID:31804486 doi:http://dx.doi.org/10.1038/s41467-019-13510-w
