6s21
From Proteopedia
Metabolism of multiple glycosaminoglycans by bacteroides thetaiotaomicron is orchestrated by a versatile core genetic locus (BT33494S-sulf)
Structural highlights
Function[ENDSF_BACTN] Endosulfatase involved in the degradation of the glycosaminoglycans (GAGs) chondroitin sulfate (CS) and dermatan sulfate (DS). Efficiently hydrolyzes sulfate groups from a broad range of substrate size, including disaccharide to high molecular weight CS and DS polymers. Has a strict specificity for the 4-O-sulfate groups of galactosamine (PubMed:25002587). GAG-specific sulfatases play a key role in the persistence of the major human gut symbiont B.thetaiotaomicron in the host gastrointestinal tract (PubMed:25002587).[1] [2] Publication Abstract from PubMedThe human gut microbiota (HGM), which is critical to human health, utilises complex glycans as its major carbon source. Glycosaminoglycans represent an important, high priority, nutrient source for the HGM. Pathways for the metabolism of various glycosaminoglycan substrates remain ill-defined. Here we perform a biochemical, genetic and structural dissection of the genetic loci that orchestrates glycosaminoglycan metabolism in the organism Bacteroides thetaiotaomicron. Here, we report: the discovery of two previously unknown surface glycan binding proteins which facilitate glycosaminoglycan import into the periplasm; distinct kinetic and genetic specificities of various periplasmic lyases which dictate glycosaminoglycan metabolic pathways; understanding of endo sulfatase activity questioning the paradigm of how the 'sulfation problem' is handled by the HGM; and 3D crystal structures of the polysaccharide utilisation loci encoded sulfatases. Together with comparative genomic studies, our study fills major gaps in our knowledge of glycosaminoglycan metabolism by the HGM. Metabolism of multiple glycosaminoglycans by Bacteroides thetaiotaomicron is orchestrated by a versatile core genetic locus.,Ndeh D, Basle A, Strahl H, Yates EA, McClurgg UL, Henrissat B, Terrapon N, Cartmell A Nat Commun. 2020 Jan 31;11(1):646. doi: 10.1038/s41467-020-14509-4. PMID:32005816[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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Categories: Bactn | Large Structures | Basle, A | Cartmell, A | Henrissat, B | Ndeh, D | Strahl, H | Terrapon, N | Cazyme | Glycobiology | Glycosaminoglycan | Gut microbiota | Hydrolase
