6s45
From Proteopedia
Room temperature structure of the dark state of the LOV2 domain of phototropin-2 from Arabidopsis thaliana determined with a serial crystallography approach
Structural highlights
FunctionPHOT2_ARATH Protein kinase that acts as a blue light photoreceptor in a signal-transduction pathway for photo-induced movements. Mediates calcium spiking of extra- and intracellular origins in response to blue light. Involved in hypocotyl phototropism. Contributes to the chloroplast accumulation in low blue light and mediates their translocation (avoidance response) at high fluence. Regulates stomata opening and photomorphogenesis response of leaf tissue. Not involved in hypocotyl elongation inhibition, anthocyanin accumulation or cotyledon opening.[1] [2] [3] [4] [5] Publication Abstract from PubMedThe recent development of serial crystallography has popularized time-resolved crystallography as a technique to determine the structure of protein-reaction intermediate states. However, most approaches rely on the availability of thousands to millions of microcrystals. A method is reported here, using monochromatic synchrotron radiation, for the room-temperature collection, processing and merging of X-ray oscillation diffraction data from <100 samples in order to observe the build up of a photoreaction intermediate species. Using this method, we monitored with a time resolution of 63 ms how the population of a blue-light photoreceptor domain in a crystal progressively photoconverts from the dark to the light state. The series of resulting snapshots allows us to visualize in detail the gradual rearrangement of both the protein and chromophore during this process. Millisecond time-resolved serial oscillation crystallography of a blue-light photoreceptor at a synchrotron.,Aumonier S, Santoni G, Gotthard G, von Stetten D, Leonard GA, Royant A IUCrJ. 2020 Jun 24;7(Pt 4):728-736. doi: 10.1107/S2052252520007411. eCollection, 2020 Jul 1. PMID:32695419[6] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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