6s6x

From Proteopedia

Structure of Azospirillum brasilense Glutamate Synthase in a6b6 oligomeric state.

6s6x, resolution 3.50Å

Structural highlights

6s6x is a 12 chain structure with sequence from Azospirillum brasilense. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.5Å
Ligands:
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GLTB_AZOBR

Publication Abstract from PubMed

Bacterial NADPH-dependent glutamate synthase (GltS) is a complex iron-sulfur flavoprotein that catalyzes the reductive synthesis of two L-Glu molecules from L-Gln and 2-oxo-glutarate. GltS functional unit hosts an alpha-subunit (alphaGltS) and a beta-subunit (betaGltS) that assemble in different alphabeta oligomers in solution. Here, we present the cryo-electron microscopy structures of Azospirillum brasilense GltS in four different oligomeric states (alpha4beta3, alpha4beta4, alpha6beta4 and alpha6beta6, in the 3.5- to 4.1-A resolution range). Our study provides a comprehensive GltS model that details the inter-protomeric assemblies and allows unequivocal location of the FAD cofactor and of two electron transfer [4Fe-4S](+1,+2) clusters within betaGltS.

Cryo-EM Structures of Azospirillum brasilense Glutamate Synthase in Its Oligomeric Assemblies.,Swuec P, Chaves-Sanjuan A, Camilloni C, Vanoni MA, Bolognesi M J Mol Biol. 2019 Aug 29. pii: S0022-2836(19)30520-0. doi:, 10.1016/j.jmb.2019.08.011. PMID:31473159^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Swuec P, Chaves-Sanjuan A, Camilloni C, Vanoni MA, Bolognesi M. Cryo-EM Structures of Azospirillum brasilense Glutamate Synthase in Its Oligomeric Assemblies. J Mol Biol. 2019 Aug 29. pii: S0022-2836(19)30520-0. doi:, 10.1016/j.jmb.2019.08.011. PMID:31473159 doi:http://dx.doi.org/10.1016/j.jmb.2019.08.011