6sh2

From Proteopedia

Crystal structure of human neprilysin E584D in complex with C-type natriuretic peptide.

Structural highlights

6sh2 is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.6Å
Ligands:	CL, NAG, ZN
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Neprilysin (NEP) is a promiscuous zinc metalloprotease with broad substrate specificity and cleaves a remarkable diversity of substrates through endopeptidase action. Two of these - amyloid-beta and natriuretic peptides - implicate the enzyme in both Alzheimer's disease and cardiovascular disease, respectively. Here, we report the creation of a catalytically inactive NEP (E584D) to determine the first peptide-bound crystal structure at 2.6 A resolution. The structure reveals key interactions involved in substrate binding which we have identified to be conserved in other known zinc metalloproteases. In addition, the structure provides evidence for a potential exosite within the central cavity that may play a critical role in substrate positioning. Together, these results contribute to our understanding of the molecular function of NEP.

Crystal structure of peptide-bound neprilysin reveals key binding interactions.,Moss S, Subramanian V, Acharya KR FEBS Lett. 2020 Jan;594(2):327-336. doi: 10.1002/1873-3468.13602. Epub 2019 Sep, 21. PMID:31514225^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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References

↑ Moss S, Subramanian V, Acharya KR. Crystal structure of peptide-bound neprilysin reveals key binding interactions. FEBS Lett. 2020 Jan;594(2):327-336. doi: 10.1002/1873-3468.13602. Epub 2019 Sep, 21. PMID:31514225 doi:http://dx.doi.org/10.1002/1873-3468.13602

1 Structural highlights
2 Publication Abstract from PubMed
3 See Also
4 References

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PDB ID 6sh2

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Categories: Homo sapiens | Large Structures | Acharya KR | Moss S | Subramanian V

6sh2

From Proteopedia

Crystal structure of human neprilysin E584D in complex with C-type natriuretic peptide.

Structural highlights

Publication Abstract from PubMed

See Also

References

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