6ssr

Structural highlights

Function

MGST2_HUMAN Catalyzes several different glutathione-dependent reactions (PubMed:8703034, PubMed:9278457, PubMed:23409838, PubMed:26656251, PubMed:26066610). Catalyzes the glutathione-dependent reduction of lipid hydroperoxides, such as 5-HPETE (PubMed:9278457, PubMed:23409838). Has glutathione transferase activity, toward xenobiotic electrophiles, such as 1-chloro-2, 4-dinitrobenzene (CDNB) (PubMed:23409838, PubMed:8703034). Catalyzes also the conjugation of leukotriene A4 with reduced glutathione to form leukotriene C4 (LTC4) (PubMed:23409838, PubMed:26656251). Involved in oxidative DNA damage induced by ER stress and anticancer agents by activating LTC4 biosynthetic machinery in nonimmune cells (PubMed:26656251).^{[1] [2] [3] [4] [5]}

See Also

• Glutathione S-transferase 3D structures

References

1. ↑ Ahmad S, Niegowski D, Wetterholm A, Haeggstrom JZ, Morgenstern R, Rinaldo-Matthis A. Catalytic characterization of human microsomal glutathione S-transferase 2: identification of rate-limiting steps. Biochemistry. 2013 Mar 12;52(10):1755-64. doi: 10.1021/bi3014104. Epub 2013 Feb, 27. PMID:23409838 doi:http://dx.doi.org/10.1021/bi3014104
2. ↑ Ahmad S, Thulasingam M, Palombo I, Daley DO, Johnson KA, Morgenstern R, Haeggstrom JZ, Rinaldo-Matthis A. Trimeric microsomal glutathione transferase 2 displays one third of the sites reactivity. Biochim Biophys Acta. 2015 Oct;1854(10 Pt A):1365-71. doi:, 10.1016/j.bbapap.2015.06.003. Epub 2015 Jun 9. PMID:26066610 doi:http://dx.doi.org/10.1016/j.bbapap.2015.06.003
3. ↑ Dvash E, Har-Tal M, Barak S, Meir O, Rubinstein M. Leukotriene C4 is the major trigger of stress-induced oxidative DNA damage. Nat Commun. 2015 Dec 11;6:10112. doi: 10.1038/ncomms10112. PMID:26656251 doi:http://dx.doi.org/10.1038/ncomms10112
4. ↑ Jakobsson PJ, Mancini JA, Ford-Hutchinson AW. Identification and characterization of a novel human microsomal glutathione S-transferase with leukotriene C4 synthase activity and significant sequence identity to 5-lipoxygenase-activating protein and leukotriene C4 synthase. J Biol Chem. 1996 Sep 6;271(36):22203-10. PMID:8703034
5. ↑ Jakobsson PJ, Mancini JA, Riendeau D, Ford-Hutchinson AW. Identification and characterization of a novel microsomal enzyme with glutathione-dependent transferase and peroxidase activities. J Biol Chem. 1997 Sep 5;272(36):22934-9. PMID:9278457
6. ↑ Thulasingam M, Orellana L, Nji E, Ahmad S, Rinaldo-Matthis A, Haeggstrom JZ. Crystal structures of human MGST2 reveal synchronized conformational changes regulating catalysis. Nat Commun. 2021 Mar 19;12(1):1728. doi: 10.1038/s41467-021-21924-8. PMID:33741927 doi:http://dx.doi.org/10.1038/s41467-021-21924-8