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Publication Abstract from PubMed

The beta-d-glucuronidase DtGlcA from Dictyoglomus thermophilum was engineered to generate an active thioglycoligase that is able to catalyse the formation of numerous S-glucuronides. Its X-ray structure analysis indicated the ability of the biocatalyst to bind aromatic thiol acceptors for S-glycosylation. Noteworthily, the DtGlcA mutant was found to be the first thioligase that is able to use a natural sugar donor different from the widely used synthetic para-nitrophenyl glycosides.

Thioglycoligation of aromatic thiols using a natural glucuronide donor.,Kurdziel M, Kopec M, Paris A, Lewinski K, Lafite P, Daniellou R Org Biomol Chem. 2020 Jul 16. doi: 10.1039/d0ob00226g. PMID:32671369^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.