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Publication Abstract from PubMed

Rishirilides are a group of PKS II secondary metabolites produced by Streptomyces bottropensis Go C4/4. Biosynthetic studies in the past have elucidated early and late steps of rishirilide biosynthesis. This work is aiming to solve the remaining steps in the rishirilide biosynthesis. Inactivation of the cyclase gene rslC3 in Streptomyces bottropensis resulted in an interruption of rishirilide production. Instead, accumulation of the tricyclic aromatic galvaquinones was observed. Similar results were observed after deletion of rslO4. Closer inspection into RslO4 crystal structure in addition to site-directed mutagenesis and molecular dynamic simulations revealed that RslO4 might be responsible for quinone formation on the third ring. The RslO1 three-dimensional structure shows a high similarity to FMN-dependent luciferase-like monooxygenases such as the epoxy-forming MsnO8 which acts with the flavin reductase MsnO3 in mensacarcin biosynthesis in the same strain. The high sequence similarity between RslO2 and MsnO3 suggests that RslO2 provides RslO1 with reduced FMN to form an epoxide that serves as substrate for RslO5.

Biosynthesis of the Tricyclic Aromatic Type II Polyketide Rishirilide: New Potential Third Ring Oxygenation after Three Cyclization Steps.,Alali A, Zhang L, Li J, Zuo C, Wassouf D, Yan X, Schwarzer P, Gunther S, Einsle O, Bechthold A Mol Biotechnol. 2021 Mar 24. pii: 10.1007/s12033-021-00314-x. doi:, 10.1007/s12033-021-00314-x. PMID:33763824^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.