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7dpd

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Human MCM9 N-terminal domain

Structural highlights

7dpd is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.55Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

MCM9_HUMAN 46,XX ovarian dysgenesis-short stature syndrome. The disease is caused by variants affecting the gene represented in this entry.

Function

MCM9_HUMAN Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR) (PubMed:23401855). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity (PubMed:26215093). Probably by regulating the localization of the MRN complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs (PubMed:23401855). Acts as a helicase in DNA mismatch repair (MMR) following DNA replication errors to unwind the mismatch containing DNA strand (PubMed:26300262). In addition, recruits MLH1, a component of the MMR complex, to chromatin (PubMed:26300262). The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression (PubMed:23401855). Probably by regulating HR, plays a key role during gametogenesis (By similarity).[UniProtKB:Q2KHI9]^[1] ^[2] ^[3]

References

↑ Park J, Long DT, Lee KY, Abbas T, Shibata E, Negishi M, Luo Y, Schimenti JC, Gambus A, Walter JC, Dutta A. The MCM8-MCM9 complex promotes RAD51 recruitment at DNA damage sites to facilitate homologous recombination. Mol Cell Biol. 2013 Apr;33(8):1632-44. doi: 10.1128/MCB.01503-12. Epub 2013 Feb, 11. PMID:23401855 doi:http://dx.doi.org/10.1128/MCB.01503-12
↑ Lee KY, Im JS, Shibata E, Park J, Handa N, Kowalczykowski SC, Dutta A. MCM8-9 complex promotes resection of double-strand break ends by MRE11-RAD50-NBS1 complex. Nat Commun. 2015 Jul 28;6:7744. doi: 10.1038/ncomms8744. PMID:26215093 doi:http://dx.doi.org/10.1038/ncomms8744
↑ Traver S, Coulombe P, Peiffer I, Hutchins JR, Kitzmann M, Latreille D, Mechali M. MCM9 Is Required for Mammalian DNA Mismatch Repair. Mol Cell. 2015 Sep 3;59(5):831-9. doi: 10.1016/j.molcel.2015.07.010. Epub 2015, Aug 20. PMID:26300262 doi:http://dx.doi.org/10.1016/j.molcel.2015.07.010