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7dtj

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Crystal structure of the RecA2 domain of RNA helicase CGH-1 in C. elegans

Structural highlights

7dtj is a 1 chain structure with sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.402Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CGH1_CAEEL Probable RNA helicase required for oocyte and sperm function. Also required to prevent the physiological germline apoptosis mechanism killing essentially all developing oocytes.^[1] ^[2]

Publication Abstract from PubMed

A protein-RNA complex containing the RNA helicase CGH-1 and a germline specific RNA-binding protein CAR-1 is involved in various aspects of function in C. elegans. However, the structural basis for the assembly of this protein complex remains unclear. Here, we elucidate the molecular basis of the recognition of CGH-1 by CAR-1. Additionally, we found that the ATPase activity of CGH-1 is stimulated by NTL-1a MIF4G domain in vitro. Furthermore, we determined the structures of the two RecA-like domains of CGH-1 by X-ray crystallography at resolutions of 1.85 and 2.40 A, respectively. Structural and biochemical approaches revealed a bipartite interface between CGH-1 RecA2 and the FDF-TFG motif of CAR-1. NMR and structure-based mutations in CGH-1 RecA2 or CAR-1 attenuated or disrupted CGH-1 binding to CAR-1, assessed by ITC and GST-pulldown in vitro. These findings provide insights into a conserved mechanism in the recognition of CGH-1 by CAR-1. Together, our data provide the missing physical links in understanding the assembly and function of CGH-1 and CAR-1 in C. elegans.

Structural and biochemical insights into the recognition of RNA helicase CGH-1 by CAR-1 in C. elegans.,Zhang Y, Lv M, Li F, Li M, Zhang J, Shi Y, Hong J Biochem Biophys Res Commun. 2021 Mar 3;549:135-142. doi:, 10.1016/j.bbrc.2021.02.119. PMID:33676181^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Navarro RE, Shim EY, Kohara Y, Singson A, Blackwell TK. cgh-1, a conserved predicted RNA helicase required for gametogenesis and protection from physiological germline apoptosis in C. elegans. Development. 2001 Sep;128(17):3221-32. PMID:11546739 doi:10.1242/dev.128.17.3221
↑ Boag PR, Nakamura A, Blackwell TK. A conserved RNA-protein complex component involved in physiological germline apoptosis regulation in C. elegans. Development. 2005 Nov;132(22):4975-86. PMID:16221731 doi:10.1242/dev.02060
↑ Zhang Y, Lv M, Li F, Li M, Zhang J, Shi Y, Hong J. Structural and biochemical insights into the recognition of RNA helicase CGH-1 by CAR-1 in C. elegans. Biochem Biophys Res Commun. 2021 Apr 16;549:135-142. PMID:33676181 doi:10.1016/j.bbrc.2021.02.119